DOES ZYMŌT SPERM SEPARATION IMPROVE EMBRYO DEVELOPMENT OUTCOMES WHEN APPLIED TO ALL INFERTILITY PATIENTS COMPARED TO DENSITY GRADIENT WASHING OR SURGICALLY ATTAINED SPERM?

The ZyMōt sperm separation device has proven favorable for use in elevated DNA fragmentation index (DFI) male factor patients, as an alternative to density gradient (DG) washing or surgically attained testicular sperm. In 2020, without fully understanding the infectivity and transmission potential of SARS-CoV2 semen, a more liberal application timed microfluidic swim-up was applied our IVF patients dilute out minimize pathogens. This study aimed evaluate whether improved normal embryo development. Retrospective analysis PGT-A/ICSI cycles (N= 3219) between 2016-2020 conducted assessed fertilization rates (FR), blastocyst development/utilization (BUR) genetic outcomes. Sperm preparations were performed per standard operating manufacturer advised (i.e., ZyMōt) procedures. Cumulus oocyte complexes harvested 36h post-hCG, stripped ICSI 3-5hr later. Zygotes at 16-18hr post-ICSI, embryos cultured under humidified tri-gas incubation up 7 days. Blastocyst (BL) development evaluated, expanded BL greater biopsied on Days 5, 6 7. All vitrified genetics determinations euploidy, aneuploidy mosaicism contrast. Applying Chi-squared analysis, we compared differences (p<0.05) oocytes inseminated by DG wash (n=23,549), (n=7,331) (n=815). No difference FR (76%), D5 formation (52-56%) BUR (52-53%) detected washed sperm, respectively. Meanwhile, had lower (70%; p<0.05), fewer forming (48%; p<0.05) overall (41%; p<0.05). addition, testicular-derived euploid (39%; with (54%; than (50%, 39%; respectively) (45%, 37%; derived-embryos. potentially viable (Euploidy+Mosiac outcome) observed groups (63-64%). Application general population offered no benefit outcomes Our data does support that using DFI is cost-effective approach attaining when ejaculated possible. However, insufficient motile morphologically are available ejaculate further needed elucidate biopsy treatment, assessments this may be biased including men non-obstructive azoospermia.